Journal: Naunyn-Schmiedeberg's Archives of Pharmacology

Article Title: The effects of melatonin on oxidative stress, inflammation, apoptosis and Nrf2/HO-1 in acrylamide-induced lung injury in rats

doi: 10.1007/s00210-025-04292-8

Figure Lengend Snippet: Lung TNF-α, IL-1β, IL-6, NF-κB, iNOS, COX-2, MPO, p38 MAPK, and IL-10 activities in experimental groups ( n = 10). Results are expressed as mean ± SEM

Article Snippet: Catalase (CAT), glutathione peroxidase (GPx), glutathione (GSH), heme oxygenase 1 (HO-1), interleukin-1 beta (IL-1β), interleukin-10 (IL-10), malondialdehyde (MDA), nuclear factor erythroid 2-related factor 2 (Nrf2), nuclear factor kappa (NF-κB), superoxide dismutase (SOD), tumor necrosis factor alpha (TNF-α), caspase 3 (CASP3), inducible nitric oxide synthase (iNOS; NOS2), interleukin-6 (IL-6), cyclooxygenase-2 (COX-2), p38 mitogen-activated protein kinases (p38-MAPK), and myeloperoxidase (MPO) enzyme-linked immunosorbent assay (ELISA) kits were purchased from BT-LAB.

Techniques:

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Silencing Ras-Related C3 Botulinum Toxin Substrate 1 Inhibits Growth and Migration of Hypopharyngeal Squamous Cell Carcinoma via the P38 Mitogen-Activated Protein Kinase Signaling Pathway

doi: 10.12659/MSM.907468

Figure Lengend Snippet: Silencing Rac1 inhibits the activation of P38 MAPK signal in vitro . The levels of MKK3 and p-MKK3 ( A, B ) and P38 and p-P38 ( C, D ) were detected by Western blot analysis. GAPDH served as the internal reference. All experiments were repeated 3 times and the results are presented as mean ±SD. *** P<0.001 compared with the negative control group.

Article Snippet: The membranes were blocked with 5% skim milk or 1% bovine serum albumin, and then incubated with Rac1 antibody, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (1: 1000; Proteintech, Wuhan, China), Cyclin D1 antibody (1: 1000; BOSTER, Wuhan, China), Cyclin E antibody, Cyclin B antibody (1: 500; Bioss, Beijing, China), metal matrix proteinase (MMP)-2 antibody, MMP-9 antibody, B cell lymphoma-2 (Bcl-2) antibody, Bcl-2-associated X protein (Bax) antibody, phosphorylated-MAP kinase (p-MKK3) antibody (1: 500; Sangon Biotech, Shanghai, China), caspase-3 antibody, caspase-9 antibody, poly ADP-ribose polymerase (PARP) antibody (1: 1000; Cell Signaling Technology, Beverly, MA, USA), p38 mitogen-activated protein kinase (MAPK) antibody, p-p38 MAPK antibody (1: 500; KeyGen, Nanjing, China), or MKK3 antibody (1: 300; BOSTER) overnight at 4°C.

Techniques: Activation Assay, In Vitro, Western Blot, Negative Control

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Silencing Ras-Related C3 Botulinum Toxin Substrate 1 Inhibits Growth and Migration of Hypopharyngeal Squamous Cell Carcinoma via the P38 Mitogen-Activated Protein Kinase Signaling Pathway

doi: 10.12659/MSM.907468

Figure Lengend Snippet: Rac1 silencing inhibits the activation of P38 MAPK signal in vivo . The levels of MKK3 and p-MKK3 ( A, B ) and P38 and p-P38 ( C, D ) in each group were detected by Western blot analysis with GAPDH as the internal reference. All experiments were repeated 3 times. The results are presented as mean ±SD. N=6. *** P<0.001 compared with the negative control group.

Article Snippet: The membranes were blocked with 5% skim milk or 1% bovine serum albumin, and then incubated with Rac1 antibody, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (1: 1000; Proteintech, Wuhan, China), Cyclin D1 antibody (1: 1000; BOSTER, Wuhan, China), Cyclin E antibody, Cyclin B antibody (1: 500; Bioss, Beijing, China), metal matrix proteinase (MMP)-2 antibody, MMP-9 antibody, B cell lymphoma-2 (Bcl-2) antibody, Bcl-2-associated X protein (Bax) antibody, phosphorylated-MAP kinase (p-MKK3) antibody (1: 500; Sangon Biotech, Shanghai, China), caspase-3 antibody, caspase-9 antibody, poly ADP-ribose polymerase (PARP) antibody (1: 1000; Cell Signaling Technology, Beverly, MA, USA), p38 mitogen-activated protein kinase (MAPK) antibody, p-p38 MAPK antibody (1: 500; KeyGen, Nanjing, China), or MKK3 antibody (1: 300; BOSTER) overnight at 4°C.

Techniques: Activation Assay, In Vivo, Western Blot, Negative Control

Journal: Stem cells and development

Article Title: p38 mitogen activated protein kinase controls two successive-steps during the early mesodermal commitment of embryonic stem cells.

doi: 10.1089/scd.2010.0213

Figure Lengend Snippet: FIG. 7. p38MAPK activity is required for 2 early successive steps in skeletal myogenesis of ES cells. EBs from wt CGR8 ES cells were differentiated in the presence or absence of PD169316 for different periods as indicated schematically in Fig. 6A. EBs were plated at day 7 and analyzed at day 26 of differentiation. (A) The ratio of differentiated EBs with spontaneous contractile myotubes was evaluated for each cell line; at least 50 different EBs per cell line were counted. Histogram shows the means SEM of 3 independent experiments. (B) In these experiments, mRNAs were extracted at day 26 and analyzed by real-time RT-PCR for expression of the specific skeletal muscle marker MyH1. Results are expressed in arbitrary units, with the values of wt CGR8 at day 26 taken as 1, and are the means SEM of at least 3 independent experiments. Significance between control and treated cells is given as *P < 0.05, **P < 0.01, and ***P < 0.001. (C), Differentiated cells at day 12 were analyzed by flow cytometry analysis of Myogenin. A histogram representative of 3 independent experiments is shown. For each experiment, control value (irrelevant) was determined as no more than 5% of positivity. (D) Differentiated cells at day 12 were analyzed by immunofluorescence using anti-MHC antibodies (magnification: 100). Nuclei were stained with DAPI. Color images available online at www.liebertonline.com=scd

Article Snippet: Membranes were incubated with antibodies against either all p38MAPK isoforms, MAPKAPK2, MAPKAPK2phospho (Cell Signalling Technology), ERK2, or HA epitope (Santa Cruz).

Techniques: Activity Assay, Quantitative RT-PCR, Expressing, Marker, Control, Cytometry, Staining

Journal: Frontiers in Pharmacology

Article Title: Computational and In Vitro Analysis of Plumbagin’s Molecular Mechanism for the Treatment of Hepatocellular Carcinoma

doi: 10.3389/fphar.2021.594833

Figure Lengend Snippet: (A) Protein expression levels of LC3-I, LC3-II, p38 MAPK, and p-p38 MAPK in SMMC-7721 cells measured by Western blot after treatment with 5 μM PL, 10 μM 3-MA autophagy inhibitor, 10 μM rapamycin autophagy agonist, 10 μM SB202190, and 10 μM SB203580 p38 MAPK inhibitor. (B) LC3-I, LC3-II, p38 MAPK, and p-p38 MAPK were used as internal reference total proteins for gray value analysis. * p < 0.05, ** p < 0.01 *** p < 0.001, compared with the PL group. # p < 0.05, ## p < 0.01 ### p < 0.001 vs. the control group. (C) Protein expression levels of LC3-I, LC3-II, p38 MAPK, and p-p38 MAPK in BEL-7404 cells measured by Western blot after treatment with 5 μM PL, 10 μM 3-MA autophagy inhibitor, 10 μM rapamycin autophagy agonist, 10 μM SB202190, and 10 μM SB203580 p38 MAPK inhibitor. (D) LC3-I, LC3-II, p38 MAPK, and p-p38 MAPK were used as internal reference total proteins for gray value analysis. * p < 0.05, ** p < 0.01 *** p < 0.001, compared with the PL group. # p < 0.05, ## p < 0.01 ### p < 0.001 vs. the control group.

Article Snippet: Plumbagin (PL) was purchased from Sigma-Aldrich (St. Louis, MO, United States) with purity ≥98%; the Acridine Orange (AO)/Ethidium bromide (EB) Double Stain Kit was from Solable Technology (Beijing, China); N-acetyl-l-cysteine, SB203580, and SB202190 were from Sigma-Aldrich (St. Louis, MO, United States); SC-79, MEK2206, 3-MA, and Z-VAD-FMK were from Selleck (Texas, United States); the BCA Protein Assay Kit, ROS Assay Kit, Annexin V-FITC Apoptosis Detection Kit and Cell lysis buffer for Western were all obtained from Beyotime Biotechnology (Shanghai, China); antibodies against Akt, phospho-Akt, mTOR, phospho-mTOR, p38 MAPK, phospho-p38 MAPK, PI3K, phospho-PI3K, LC3B, cleave-RP, and cleave-caspase 3 were from Cell Signaling Technology, Inc. (Boston, MA, United States); and β-p38 MAPK was purchased from Boster Technology (Wuhan, China).

Techniques: Expressing, Western Blot, Control

Journal: Materials

Article Title: A Novel Bioactive Endodontic Sealer Containing Surface-Reaction-Type Prereacted Glass-Ionomer Filler Induces Osteoblast Differentiation

doi: 10.3390/ma13204477

Figure Lengend Snippet: ( A ) mRNA expression of ALP and IBSP induced by set PRG+ extract significantly downregulated by application of NPS2143 (CaSR antagonist) in Kusa-A1 cells; * p < 0.05. ( B ) Phosphorylation of extracellular signal-regulated kinase (ERK) and p38 promoted by set PRG+ extract is downregulated by the application of NPS2143. Phosphorylation of ERK and p38 promoted by Sr 2+ is also downregulated by NPS2143 application. α-Tubulin was used as loading control. Experiments were repeated three times with similar results.

Article Snippet: Membranes were incubated with 1:1000-diluted polyclonal antibodies against extracellular signal-regulated kinase (ERK), phosphorylated ERK (pERK; 1:1000; BD Bioscience, San Jose, CA USA), p38 mitogen-activated protein kinase (MAPK), and phosphorylated p38 MAPK (pp38; BD Bioscience) overnight at 4 °C.

Techniques: Expressing